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rat anti zo 1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rat anti zo 1
    Rat Anti Zo 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+zo+1/ZO-1+Antibody/bio_rxiv__64898__2026__03__20__713242-258-36-39
    Average 96 stars, based on 1122 article reviews
    rat anti zo 1 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: The Joubert syndrome protein CSPP1 is a conserved regulator of vertebrate multiciliogenesis and motile cilia function
    Article Snippet: .. Primary antibodies used for MTEC, trachea, and ependyma immunofluorescence were mouse anti-Centrin (clone 20H5, 1:500, EMD Millipore 04-1624), rabbit anti-Cep164 (1:1000, Proteintech 22227-1-AP), mouse anti-acetylated α-tubulin (clone 6-11B0-1, 1:2000, Sigma T6793), rabbit anti-DEUP1 (1:200, Sigma HPA010986), rat anti-ZO-1 (1:1000, Santa Cruz sc-33725), mouse anti-γ-tubulin (GTU-88, 1:1000, Sigma-Aldrich T6557), rabbit anti-CSPP1 (1:50, Proteintech 11931-1-AP), mouse anti-ODF2 (1:200, Novus/Abnova H00004957-M01), mouse anti-PCM1 (G6 clone, 1:500, Santa Cruz sc-398365), and mouse anti-α-tubulin (DM1A, 1:500, Sigma T926). .. Confocal imaging of MTECs and trachea was performed on Leica TCS SP8 and Leica Stellaris 8 inverted laser-scanning confocal microscopes equipped with HC PL APO CS2 oil-immersion objectives (63×/1.4 NA or 40×/1.3 NA).

    Article Title: E- and N-cadherin drive hepatic polarity and lumen elongation via opposing effects on RhoA activity
    Article Snippet: .. Indirect immunofluorescence analysis was performed using the following primary antibodies: mouse anti-E-cadherin (BD Biosciences, 610182, 1:200), rabbit anti-E-cadherin (GeneTex, GTX100443, 1:200), mouse-anti-N-cadherin (BD Biosciences, 610921, 1:200), mouse anti-N-cadherin (Santa Cruz Biotechnology, sc-59987, 1:200), rabbit anti-N-cadherin (Cell Signaling Technology, 13116, 1:250), rat anti-ZO-1 (Santa Cruz Biotechnology, sc-33725, 1:500), rabbit anti-ZO-1 (Thermo Fisher Scientific, 40-2200, 1:2000), mouse anti-Mdr1 (Thermo Fisher Scientific, MA1-26528, 1:100), rabbit anti-radixin (GeneTex, GTX105408, 1:250), mouse anti-aPKC (Santa Cruz Biotechnology, sc-17781, 1:200), rabbit anti-pericentrin (abcam, ab220784, 1:1000), rabbit anti-Par3 (Millipore, 07-330, 1:1000), mouse anti-NuMA (Millipore, MABE1807, 1:200), rabbit anti-EPB41L2/4.1G (abcam, ab175928, 1:250), mouse anti-RhoA (Santa Cruz Biotechnology, sc-418, 1:200), mouse anti-p120-catenin (Santa Cruz Biotechnology, sc-23872, 1:200), rabbit anti-ARVCF (Thermo Fisher Scientific, PA5-64129, 1:200), rabbit anti-ROCK2 (Cell Signaling Technology, 47012, 1:200). .. The secondary antibodies and phalloidin used were: Alexa Fluor 405- conjugated goat anti-rat IgG antibodies (abcam, ab175671), Alexa Fluor 488-conjugated chicken anti-rabbit (Thermo Fisher Scientific, A21441) or goat anti-mouse IgG antibodies (Thermo Fisher Scientific, A11001), Alexa Fluor 555-conjugated goat anti-rabbit (Thermo Fisher Scientific, A21428) or goat anti-mouse IgG antibodies (Thermo Fisher Scientific, A21422), and Alexa Fluor 647-conjugated phalloidin (Thermo Fisher Scientific, A22287).

    Incubation:

    Article Title: Mice with renal-specific alterations of stem cell-associated signaling develop symptoms of chronic kidney disease but surprisingly no tumors.
    Article Snippet: For haematoxylin-eosin (H&E) staining, sections were rehydrated and stained with haematoxylin (Fluka) for 4 min and with eosin (Merck) for 2 min. For immunofluorescence, sections were rehydrated, and antigen retrieval in TRIS-EDTA and blocking with 10% donkey serum (Bio-Rad) were performed. .. Sections were incubated overnight with rat anti-BrdU (1:100, Abcam, ab6326), mouse anti-E-cad (1:300, BD Biosciences, 610181), rabbit anti-Laminin (1:500, Abcam, ab11575), rat anti-Zo-1 (1:200, Santa Cruz, sc-33725), mouse anti-MDR1 (1:50, Santa Cruz, sc-55510) and mouse anti-AQP3 (1:50, Santa Cruz, sc-518001) primary antibodies or with Biotinylated LTL (1:300, Vector Laboratories, B-1325) and Rhodamine-conjugated PNA (1:300, Vector Laboratories, RL-1072) lectins, followed by incubation with donkey fluorescent dye-conjugated Alexa488 anti-mouse, Alexa488 anti-rabbit, Alexa488 anti-rat and Cy3 anti-rat (1:250, Jackson) secondary antibodies or with Alexa647-conjugated Streptavidin (1:200, Thermo Fisher Scientific) and by counterstaining with DAPI (0.2 μg/ml, Thermo Fisher Scientific) for 1 hr. .. For immunohistochemistry, sections were rehydrated, antigen retrieval in TRIS-EDTA was performed, endogenous peroxidase activity was blocked in 3.5% (v/v) hydrogen peroxide and blocking with 10% donkey serum (Bio-Rad) was performed.

    Article Title: Lactobacillus rhamnosus GG and Tannic Acid Synergistically Promote the Gut Barrier Integrity in a Rat Model of Experimental Diarrhea via Selective Immunomodulatory Cytokine Targeting.
    Article Snippet: Scope: Diarrhea is a common health issue that contributes to a significant annual death rate among children and the elderly worldwide.. The anti-diarrheal activity of Lactobacillus rhamnosus GG (LGG) and tannic acid (TA), alone or combined, is examined, in addition to their effect on intestinal barrier integrity.. Methods and results: Fifty-six adult male Wistar rats are randomly assigned into seven groups: control, LGG alone, TA alone, diarrhea model, diarrhea+LGG, diarrhea+TA, and diarrhea+LGG+TA-treated groups.

    Article Title: Transcriptomic comparison of corneal endothelial cells in young versus old corneas
    Article Snippet: .. Overnight incubation at 4 °C was performed with one of several antibodies: rat anti-ZO-1 (sc-33725, Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. After washing with PBS, samples were incubated with fluorescein isothiocyanate (FITC)-conjugated goat anti-rat IgG (1:100) for 2 h at 25 °C in darkness, followed by counterstaining with Hoechst 33,342 nuclear staining dye (1:2000; Molecular Probes, Eugene, OR, USA).

    Article Title: COL8A2 Activation Enhances Function of Corneal Endothelial Cells through HIPPO Signaling/Mitochondria Pathway.
    Article Snippet: Corneal endothelial cells (CECs) are essential for maintaining corneal transparency and hydration through their barrier and pump functions.. The COL8A2 gene encodes a component of the extracellular matrix of the cornea, which is crucial for the normal functioning of these cells.. Mutations in COL8A2 are linked to corneal dystrophies, emphasizing the gene’s importance in corneal health.

    Article Title: Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial.
    Article Snippet: .. The sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: rabbit anti-CD31 (1:100, GB11063-2, RRID:AB_2922436, Serivicebio, China) and rat anti-occludin (1:100, #68534, RRID:AB_3683657, CST, AC EPTED MANUSCRIPTARTICLE IN PRESS AR TIC LE IN PR ES S Ac ce pt ed m an us cr ip t ACCEPTED MANUSCRIPT USA), rat anti-ZO-1 (1:100, sc-33725, RRID:AB_628459, Santa Cruz, USA). ..

    Article Title: Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial Autophagy
    Article Snippet: .. The sections were incubated overnight at 4 °C with the following primary antibodies diluted in blocking solution: rabbit anti-CD31 (1:100, GB11063-2, RRID:AB_2922436, Serivicebio, China) and rat anti-occludin (1:100, #68534, RRID:AB_3683657, CST, USA), rat anti-ZO-1 (1:100, sc-33725, RRID:AB_628459, Santa Cruz, USA). ..

    Blocking Assay:

    Article Title: Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial.
    Article Snippet: .. The sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: rabbit anti-CD31 (1:100, GB11063-2, RRID:AB_2922436, Serivicebio, China) and rat anti-occludin (1:100, #68534, RRID:AB_3683657, CST, AC EPTED MANUSCRIPTARTICLE IN PRESS AR TIC LE IN PR ES S Ac ce pt ed m an us cr ip t ACCEPTED MANUSCRIPT USA), rat anti-ZO-1 (1:100, sc-33725, RRID:AB_628459, Santa Cruz, USA). ..

    Article Title: Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial Autophagy
    Article Snippet: .. The sections were incubated overnight at 4 °C with the following primary antibodies diluted in blocking solution: rabbit anti-CD31 (1:100, GB11063-2, RRID:AB_2922436, Serivicebio, China) and rat anti-occludin (1:100, #68534, RRID:AB_3683657, CST, USA), rat anti-ZO-1 (1:100, sc-33725, RRID:AB_628459, Santa Cruz, USA). ..



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